解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Quantitative nuclear morphometry (QNM) provides a reliable, reproducible method for measuring the degree of nuclear pleomorphism, a qualitative parameter widely incorporated into histopathological grading schemes. Nuclear shape has successfully been shown to predict primary prostate adenocarcinoma metastatic potential...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.3.673
更新日期:1995-09-01 00:00:00
abstract::The purpose of this study was to determine whether DU145, a human prostate cancer cell line: (a) transcribes mRNAs coding for beta A- and beta B-subunits of activin, a member of transforming growth factor beta (TGF beta) superfamily, and activin receptors I, II, and IIB; and (b) produces activin proteins. The expressi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.2.239
更新日期:1995-08-01 00:00:00
abstract::With a view to identifying markers reflecting not the evidence of, but the potential for, neoplastic progression of the breast we have compared the normal invasive mammary epithelial cell growth seen at puberty with invasive and non-invasive carcinogenesis using the mouse as a model. We have analyzed cell proliferatio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::The peptide hormone, GnRH has been hypothesized to play a direct antitumoral role in certain kinds of breast cancer. This direct effect is considered to function via high affinity membrane receptors to GnRH that have been demonstrated in tumors. However, no routine assay for GnRH receptors exist limiting further resea...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Highly aggressive murine B16 melanoma was engineered to secrete IFN-alpha constitutively. Cells expressing IFN-alpha were injected into syngeneic C57BL/6 mice and the mice were monitored for tumor development. Secretion of IFN-alpha by B16 melanoma cells completely abrogated their tumorigenicity in syngeneic mice. LFN...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.1.17
更新日期:1995-07-01 00:00:00
abstract::Selectivity of the anticancer agent PB-100 for malignant cells, already demonstrated using cell growth and viability evaluation, is now confirmed by microscopic observations. PB-100 is easily detected inside cells by its yellow color under visible light and by its blue fluorescence; it may be measured in isolated nucl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.1.81
更新日期:1995-07-01 00:00:00
abstract::A total of forty-one tumor infiltrating T cell lines (TIL) were cloned, in the presence of interleukin-2, from nine breast tumor and five melanoma specimens with limiting dilution in a microculture system. Nineteen (46%) of the lines/clones reacted to autologous tumor targets. The T cell receptor (TcR) V beta gene usa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.1.147
更新日期:1995-07-01 00:00:00
abstract::According to the current concept of carcinogenesis, neoplastic transformation consists of multistep accumulations of adverse genetic and epigenetic events. Recent advances in molecular genetics have demonstrated aberrations of oncogenes and tumor-suppressor genes in a variety of human cancers. The loss of wild-type p5...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.6.1209
更新日期:1995-06-01 00:00:00
abstract::The extracellular cGMP levels or the ratio between extracellular levels of cGMP and cAMP (cGMP(ex)/cAMP(ex)) have been proposed as tumor marker for premalignant and malignant diseases of the uterine cervix. More than 50% of cervical cancers occur in premenopausal women and detailed information about hormonal and drug ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.6.1279
更新日期:1995-06-01 00:00:00
abstract::Marek's disease virus (MDV) is one of the most oncogenic herpesviruses and the only cancer virus that has a successful vaccine. Based on the short-latency and polyclonal nature of the tumors, it is likely that MDV encodes its own oncogenes. Recent studies have provided insights into possible MDV genes involved in onco...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.5.997
更新日期:1995-05-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF), a secreted endothelial-specific growth factor, and microvessel density was examined in 204 primary non-small cell lung carcinomas. The expression of VEGF was evaluated by immunohistochemical staining using an anti-VEGF antibody. Positive staining was obtained in 125 out of 20...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.5.1059
更新日期:1995-05-01 00:00:00
abstract::The tumor suppressor protein p53 is one of the most important manipulators of the cell cycle. By interaction with targets such as WAF1/CIP1, mdm2 or GADD45 p53 functions as a negative or positive regulator of the cell cycle. After DNA damage p53 is implicated in growth arrest of cells in the G(1) phase of the cell cyc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.5.1129
更新日期:1995-05-01 00:00:00
abstract::Using two malignant rat glioma cell lines, we tested to what extent peripheral immunization could affect tumor growth in the brain of syngeneic rats. Peripheral subcutaneous (s.c.) immunization was performed with autologous Newcastle-disease-virus (NDV)-infected or non-infected live tumor cells. Thus immunized rats or...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.4.759
更新日期:1995-04-01 00:00:00
abstract::In four previous case-control studies we have shown an association between exposure to phenoxyacetic acids or chlorophenols and soft-tissue sarcoma (STS). These chemicals are contaminated by dioxins and dibenzofurans, some of which are known carcinogens in experimental animals. In a meta-analysis of our four studies w...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.4.847
更新日期:1995-04-01 00:00:00
abstract::Dose is a critical determinant of the efficacy of chemotherapy. The dose response curve for virtually all anticancer agents is steep in both in vitro and in vivo experimental studies. However the application of the steep dose-response curve in the clinic has been troublesome for many years because of dose limiting bon...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Tissue samples from a malignant melanoma of the anal canal and its local metastasis were short-term cultured and analyzed cytogenetically. Complex chromosome aberrations were found in the primary tumor, yielding the karyotype 49-50,X,-Y,der(1)t(1;13)(q44;q12),+der(2)t(2;8) (q31;q12),der(6)t(5;6)(q13;q21),+del(6)(q12q2...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.3.555
更新日期:1995-03-01 00:00:00
abstract::The epidermal growth factor (EGF) receptor (EGFR) is activated by EGF and other EGF-like growth factors, including amphiregulin (AR). We characterized the localization and mitogenic action of AR in T3M4 and COLO-357 human pancreatic cancer cell lines and determined whether the presence of AR in human pancreatic cancer...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.3.625
更新日期:1995-03-01 00:00:00
abstract::Five retinoblastoma tumours from patients treated in the King Khaled Eye Specialist Hospital in Riyadh were analysed for mutations in the RB1 gene using single strand conformation polymorphism analysis and mutations were found in all of them. In one tumour the mutation in exon 11 was homozygous demonstrating the role ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.3.687
更新日期:1995-03-01 00:00:00
abstract::Tumor cell (TC) metastasis is initiated by selective adhesion of TCs to target structures such as basement membrane and endotheliaI cells (ECs), followed by transvascular migration of TCs. Variants of murine B16 melanoma having different metastatic potentials (in the order BL6 greater than or equal to F10>F1>WA4) have...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.2.319
更新日期:1995-02-01 00:00:00
abstract::The retinoic acid receptor (RAR) beta gene is located in a region on chromosome 3p, which is frequently deleted in renal cell carcinoma. Since retinoic acid (RA) can inhibit cell proliferation and tumor formation, loss of RAR beta might contribute to oncogenesis of the kidney. This prompted us to examine RAR beta expr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.2.391
更新日期:1995-02-01 00:00:00
abstract::To determine the tissue specificity of the potential mutations in DNA polymerase beta (pol beta), alterations in the pol beta were examined in breast, prostate, and colorectal primary tumors. The pol beta is known to contribute to mammalian base excision repair and replication. Similar to colorectal cancer, a high occ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::Three separate experiments, designed to minimize the DNA repair process following exposures to mutagens, were performed to determine whether DNA repair capability is the sole factor responsible for differential mutagen sensitivity. These experiments included (i) very short bleomycin pulse treatments (2, 5 and 10 min),...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.51
更新日期:1995-01-01 00:00:00
abstract::Loss of heterozygosity (LOH) or allelic deletion at various loci has been reported in the majority of human tumors. The frequently deleted targets are believed to be tumor suppressor genes. Recent studies have identified the APC and MCC genes at 5q21, as putative tumor suppressor genes. The APC and MCC genes have been...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.111
更新日期:1995-01-01 00:00:00
abstract::The neuroepithelioma cell line CHP100 expresses low but detectable amounts of N-myc protein together with large amounts of c-myc protein. We have recently demonstrated that antisense inhibition of N-myc expression in CHP100 cells leads to decreased in vitro growth and alterations in cellular morphology without affecti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.175
更新日期:1995-01-01 00:00:00
abstract::In this communication we show that overexpression of the human TATA-binding protein (TBP) activates a synthetic promoter with p53-binding sites in the presence of wild-type p53. No activation is observed in the absence of wild-type p53 in Saos-2 cells. Perhaps, TBP is limiting in these cells for the p53-mediated activ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::In this study we examined the levels of IL-1 alpha, IL-1 beta, IL-2, IL-6, TNF-alpha and sIL-2R in the sera and culture supernatants of PHA-stimulated lymphocytes from a series of 90 cancer patients. The expression of the IL-2R p55 chain (alpha subunit) on PHA-stimulated lymphocytes was also evaluated together with th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.6.1211
更新日期:1994-12-01 00:00:00
abstract::The murine double minute-2 (MDM2) gene encodes a 90 kDa protein which binds and inactivates the protein product of the p53 tumor suppressor gene. To elucidate the potential role of MDM2 in benign and malignant hyperproliferative conditions in the pancreas, we studied MDM2 expression in cultured human pancreatic cancer...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.6.1279
更新日期:1994-12-01 00:00:00
abstract::We have analyzed proteins produced in Various human gastric carcinoma cell lines and normal gastric mucosa. The production of HSP90 increased in eight gastric carcinoma cell lines. Gelsolin content was down-regulated in seven gastric carcinoma cell lines. Furthermore, the production of GST-pi increased in a poorly dif...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.6.1347
更新日期:1994-12-01 00:00:00
abstract::The human teratocarcinoma cell line (Tera 2) could be stimulated to a moderate increase in cell number in serum-free medium by addition of 5 ng leukemia inhibitory factor (LIF)/ml. However this effect was only observed in short term (24 h) cultures. By comparing cell numbers with thymidine incorporation data and propo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.6.1419
更新日期:1994-12-01 00:00:00
abstract::Changes in the expression of the putative p53-inducible inhibitor p21 of cyclin-dependent kinase (CDK) and p21-associated CDK activity were examined in relation to the DNA synthesis-associated nuclear exclusion of p53 after stimulating growth arrested human breast cancer MCF7 cells with serum. Following stimulation, p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.5.1059
更新日期:1994-11-01 00:00:00
abstract::A murine B16 melanoma parental line (B16) and two clones either pigmented (B16P) or non-pigmented (B16NP) were cocultured as aggregates with mouse 3T3 fibroblasts. In vivo, these mixed aggregates developed more aggressive tumors in the mouse than corresponding pure aggregates. In vitro, the three pure cell lines secre...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.5.1125
更新日期:1994-11-01 00:00:00
abstract::Human mammary and thyroid tumors as well as peripheral blood cells of the same patients were examined for the amplification of oncogene fins and the expression of its product. Of the 7 mammary tumors analyzed, amplification of fms was observed in 6 (86%) mammary tumor DNAs, while no amplification was seen in 5 thyroid...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.4.769
更新日期:1994-10-01 00:00:00
abstract::Thirty-four children with newly diagnosed acute nonlymphoblastic leukemia were analysed for the expression of P-glycoprotein (P-170), glutathione S-transferase-pi, (GST-pi) topoisomerase II (Topo II) and the proliferation antigen Ki-67 by the streptavidin-biotin-peroxidase method. Overexpression of P-170 was present i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.4.841
更新日期:1994-10-01 00:00:00
abstract::In a retrospective analysis of 91 consecutive oral carcinomas we reviewed all patient records and weighed clinicopathological parameters against the results of image DNA cytometry and p53 immunhistochemical staining of the initial diagnostic biopsies. Eighty-seven percent of the biopsies were either aneuploid or nondi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.4.915
更新日期:1994-10-01 00:00:00
abstract::The effect of immune cells on the growth and development of human prostatic cancer cells was investigated. Cell proliferation of androgen-independent human prostatic cancer cells JCA-1 was reduced 30-40% with the supplementation of a growth modulating activity present in lymphocyte conditioned medium. The slower growi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.4.979
更新日期:1994-10-01 00:00:00
abstract::HER-2/neu status and t-PA, u-PA, and PAI-1 cytosol content were evaluated in 88 primary breast cancer patients to determine the relationships between these parameters. HER-2/neu was amplified in 24% (20/84) of tumor samples and overexpressed in 28% (14/50). In the overall series, median t-PA, u-PA and PAI-1 contents, ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Sixty-four colorectal carcinomas were evaluated for allelic loss of chromosomes 17p and 5q, as well as point mutation of the p53 tumor suppressor gene. Allelic loss of chromosomes 5q and 17p were found to be weakly associated, whereas point mutation of the p53 gene was found to be more strongly associated with chromos...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.3.539
更新日期:1994-09-01 00:00:00
abstract::The regions upstream from the human N-myc gene were examined for both transcription and replication. The plasmids containing several N-myc regions linked to the bacterial chloramphenicol acetyltransferase (CAT) gene were transfected to human neuroblastoma IMR32 cells. The results indicated that the region spanning fro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.3.603
更新日期:1994-09-01 00:00:00
abstract::Low concentrations of monoclonal antibodies (mAb) specific for the CD3 component of the T cell receptor complex (TcR) may be both immunosuppressive or potent activators of T cell function in vitro depending upon the experimental situation. The anti-CD3 mAb OKT3 is imnmunosuppressive at high doses (5 mg/day for 10 to 1...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.3.675
更新日期:1994-09-01 00:00:00
abstract::Peripheral blood mononulear cells (PBMC) derived from 10 asymptomatic human T lymphotropic virus type I (HTLV-I) carriers were cultured for a short term (10-14 days) in the absence of exogenous antigens. In 5 carriers, when compared with 5 HTLV-I non-carriers an apparent increase in the proportion of CD8+ DR+ cells wa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.2.159
更新日期:1994-08-01 00:00:00